Synthesis of N-Ethyl-2-[1-(2-pyrazinyl) ethylidene] hydrazinecarbothioamide (APZ-ETSC) and Characterization by NMR Spectroscopy

  • Dylan Gardner


The synthesis of a new molecule, acetylpyrazine ethylthiosemicarbazone (APZ-ETSC) is presented and its structural characterization is described with NMR techniques using experiments from a 500 MHz Bruker Nuclear Magnetic Resonance (NMR) Spectrometer. The 1H, 13C-Distortionless Enhancement by Polarization Transfer (DEPT), 1H-13C Heteronuclear Single Quantum Coherence (HSQC), 1H-13C Heteronuclear Multiple Bond Coherence (HMBC), 1H-15N HSQC, and 1H-15N HMBC experiments were conducted with the NMR spectrometer, and the chemical shifts obtained by these experiments were correlated to confirm the assignments of the spectral peaks of the new molecule. These NMR experiments give evidence for the hypothesized structure of APZ-ETSC in solution.